I can't pinpoint the exact revision since after 409, there was a bug that causes games to crash when transitioning to new areas. But since that bug got fixed, a new one popped up in Resident Evil 4 that wasn't in 4.0 409. When you enter the fly cave after beating the the two El Gigentes, the game freezes when you enter one of the smaller caves that has a button to activate the light to open a door.
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